Ins2-cre銆丷IP-cre
2022-11-19
B6.Cg-Tg(Ins2-cre)25Mgn
鍝佺郴绶ㄨ櫉锛�GAP1051
鍝佺郴鐗归粸(di菐n)锛�
RIP-Cre 杞�(zhu菐n)鍩哄洜鍏锋湁澶ч紶鑳板扯绱� II 鍟熷嫊瀛�銆佹牳瀹氫綅 Cre 閲嶇祫閰剁殑 668 bp 鐗囨鍜屼締鑷汉椤炵敓闀锋縺绱犲熀鍥犵殑 2.1 kbp 鐗囨銆傛敎甯堕€欑ó杞�(zhu菐n)鍩哄洜鐨勫崐鍚堝瓙灏忛紶鍦ㄨ〃鍨嬩笂鏄甯哥殑骞朵笖閬庤〃閬�(d谩)cre鐗瑰垾鏄湪鑳拌吅尾绱�(x矛)鑳炰腑銆傞€欑ó杞�(zhu菐n)鍩哄洜鑿屾牚鑸囨敎甯� floxed 闈跺悜绐佽畩鐨勫皬榧犵祼(ji茅)鍚堜娇鐢�锛屼娇鐢ㄢ€�Cre-lox鈥濈郴绲�(t菕ng)鍓�(chu脿ng)寤哄悇绋儼鑵� 尾 绱�(x矛)鑳炵壒鐣版€у熀鍥犳暡闄�銆備締鑷咕鍊�(g猫)涓嶅悓瀵�(sh铆)椹�(y脿n)瀹ょ殑绲�(ji茅)鏋滆〃鏄�锛岄€欑ó杞�(zhu菐n)鍩哄洜鑿屾牚鍦ㄥ(sh铆)鐝�(xi脿n)鑳拌吅 尾 绱�(x矛)鑳炵壒鐣版€ч噸绲勬柟闈㈣嚦灏戞湁 85% 鐨勬晥鐜�銆�
閭勬噳(y墨ng)娉ㄦ剰锛屽凡鐧�(f膩)鐝�(xi脿n)瑭茬郴涓殑杞�(zhu菐n)鍩哄洜鍦ㄤ笅涓樿叇涓互浣庢按骞宠〃閬�(d谩)銆傚湪鏌愪簺鎯呮硜涓�锛岀敱浜庡ぇ鑵﹁┎鍗€(q奴)鍩熶腑floxed绛変綅鍩哄洜鐨勭己澶�锛岄€欏皫(d菐o)鑷翠簡涓€绋〃鍨�銆傞倓琛ㄦ槑锛岄€欎簺杞�(zhu菐n)鍩哄洜灏忛紶鍙兘鏈冨湪 6-8 鍛ㄩ健鏅�(sh铆)鑷櫦(f膩)鐧�(f膩)灞曠偤钁¤悇绯栬€愬彈涓嶈壇鍜岃儼宄剁礌鍒嗘硨鍙楁悕銆�
閬哄偝瀛�(xu茅)淇℃伅锛�
閬哄偝鑳屾櫙锛�C57BL/6J
鍝佺郴椤炲瀷锛�杞�(zhu菐n)鍩哄洜
鐩搁棞(gu膩n)鍩哄洜锛�Ins2-cre
椋奸(y菐ng)淇℃伅锛�
閰嶇箒绛栫暐锛�
Homozygote x Homozygote
閰嶇箒鐗规€э細
鐣�(d膩ng)缍寔绋兢鏅�(sh铆)锛屼竴鑸彲浠�绱斿悎瀛�閫�(j矛n)琛屼繚绋�銆�
鎳�(y墨ng)鐢ㄩ牁(l菒ng)鍩燂細
绯栧翱鐥呭拰鑲ヨ儢鐮旂┒銆�
鍙冭€冩枃鐛�(xi脿n)锛�
1. https://www.jax.org/strain/003573
