Gcgcre:GFP
2022-11-19
B6.Cg- Gcg tm1.1(cre/GFP)Rpa
鍝佺郴绶ㄨ櫉锛�GAP1090
鍝佺郴鐗归粸锛�
Cre 閲嶇祫閰剁敱灏忛紶Gcg 锛堣儼楂樿绯栫礌锛夊暉鍕曞瓙琛ㄩ仈銆傜暥鑸囨敎甯舵劅鑸堣叮鐨�loxP鍋�(c猫)缈煎簭鍒楃殑灏忛紶闆滀氦鏅�锛屽湪瀛ゆ潫鏍�(NTS)鐨勮〃閬�Gcg鐨勭窗鑳炰腑鍒囬櫎floxed鍗€(q奴)鍩�銆�
鏍稿畾浣嶄俊铏� (NLS) 灏� cre 鐨勮〃閬斿紩灏庤嚦绱拌優鏍�銆�Myc-tag 鍔熻兘灏氭湭缍�(j墨ng)閬庢脯瑭︺€傞洊鐒剁磵鍏ヤ簡闈跺悜绲�(ji茅)妲�(g貌u)锛屼絾 GFP 鐔掑厜澶綆锛岀劇娉曠洿鎺ユ垨閫氶亷鍏嶇柅绲勭箶鍖栧瑙€瀵�銆�
瑭查洔鍚堝皬榧犳槸鍙鐨�銆佸彲鐢熻偛鐨勶紝骞朵笖鐢变簬鍩哄洜鐨勭法纰煎崁(q奴)涓嶅彈褰遍熆锛屽洜姝ゆ矑鏈夎〃鐝�(xi脿n)鍑烘槑椤殑琛ㄥ瀷銆�
閬哄偝瀛镐俊鎭細
閬哄偝鑳屾櫙锛�C57BL/6J
鍝佺郴椤炲瀷锛�杞�(zhu菐n)鍩哄洜
鐩搁棞(gu膩n)鍩哄洜锛�cre/GFP
椋奸(y菐ng)淇℃伅锛�
閰嶇箒绛栫暐锛�
Heterzygote x Wildtype or Wildtype x Heterzygote
閰嶇箒鐗规€э細
鐣剁董鎸佺ó缇ゆ檪锛屼竴鑸彲浠ラ洔鍚堝瓙閫茶淇濈ó銆�
鎳�(y墨ng)鐢ㄩ牁(l菒ng)鍩燂細
鑳拌吅伪绱拌優鐗圭暟鎬ц〃閬�銆�
鍙冭€冩枃鐛伙細
1. https://www.jax.org/strain/030542
